Disinfectant Testing

Perfectus Biomed offer a range of planktonic and biofilm disinfectant testing. Our testing can be used to ascertain the activity of antimicrobial agents under specified conditions. We work with clients to generate evidence in support of products that consistently remove or inactivate pathogens. Our disinfectant testing can be carried out on inanimate surfaces with and without interfering substances. Our team of PhD microbiologists are available to discuss method adaptations or to custom design disinfectant testing that meets with your requirement.

Perfectus Biomed disinfectant testing includes carrier tests, suspension tests, capacity testing, practical and in use testing. We offer these tests in line with standard test methods including; BS EN 1040, BS EN 1276, BS EN 1499, BS EN 1500, BS EN 1650, BS, EN 1656, BS EN 1657, BS EN 13727, BS EN 13624, BS EN 13697, BS EN 13704, BS EN 14476, BS EN 14675, BS EN 14561, ASTM E2197-11, ASTM E2414-05. If you do not see the disinfectant test you are looking for please call us to discuss your requirements further.

Our disinfectant testing can be used to support infection control in healthcare settings and in the community. Microorganisms can be spread by direct and indirect contact and can persist on hard surfaces for prolonged periods of time. Microorganisms such as VRE can persist on a surface for as long as 4 months, Pseudomonas aeruginosa and E. coli can persist for 16 months and Klebsiella spp. have demonstrated up to 30 months of persistence on an inanimate object (Kramer A, Schwebke I, Kampf G. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis. 2006;6:130.) Disinfectant manufacturers make claims around their antimicrobial activity relating to time of kill, persistence on a surface and residual activity.

Traditional disinfectant testing focuses on planktonic isolates that are typically easier to kill and less persistent than multispecies biofilm encased microorganisms. We recognise that, in nature, an estimated 80% of microorganisms exist within polymicrobial biofilms. With this in mind we design test methodologies that produce meaningful data.

Experts at Perfectus Biomed will work with you to design test methods that replicate ‘real life scenarios’ that demonstrate the efficacy of disinfectant products against potential pathogenic microorganisms, including those that exist within a microbial biofilm.

Our customised test methodologies include:

  • EpiDerm™ skin corrosion test model
  • Minimum inhibitory concentration determination
  • Minimum eradication concentration determination
  • Minimum biofilm eradication concentration determination
  • Algae resistance test based on ASTM G29
  • Custom antimicrobial hard surface testing
  • Room fogging studies
  • Zone of inhibition
  • Residual activity assays

Test products include:

  • Household disinfectants
  • Clinical disinfectants
  • Hand sanitisers
  • Hard surface wipes
  • Fogging devices
  • Aerosols
  • Other – please contact us to discuss.

Perfectus Surface Antimicrobials (UK)

We also offer a range of standard test methodologies including:

  • BS EN 1040: Basic bactericidal activity of chemical disinfectants
  • BS EN 1276: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • BS EN 1499: Chemical disinfectants and antiseptics. Hygienic hand wash. Test method and requirements.
  • BS EN 1500: Chemical disinfectants and antiseptics. Hygienic hand rub. Test method and requirements.
  • BS EN 1650: Quantitative suspension test for evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
  • BS EN 1656: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 1657: Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 13727: Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants for instruments used in the medical area.
  • BS EN 13624: Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity in the medical area.
  • BS EN 13697: Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas.
  • BS EN 13704: Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas.
  • BS EN 14476: Viricidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine.
  • BS EN 14675: Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area.
  • BS EN 14561: Quantitative carrier test for the evaluation of bactericidal activity for instruments used in the medical area.
  • ASTM E2197-11: Standard quantitative disk carrier test method for determining the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activities of liquid chemical germicides.
  • ASTM E2414-05: Standard test method for quantitative sporicidal three step method to determine sporicidal efficacy of liquids, liquid sprays, and vapour or gases on contaminated carrier surfaces.

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii, Actinomyces naeslundii, Adenovirus (human), Aspergillus brasiliensis, Aspergillums niger, Aureobasidium pullulans, Bacillus cereus, Bacillus subtilis, Camplylobacter jejuni, Candida albicans, Clostridium difficile, Corynebacterium minutissimum, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium (VRE), Enterococcus hirae, Escherichia coli, Fusobacterium nucleatum, Haemophilus influenzae, Heliocbacter pylori, Halomonas magadiensis, Klebsiella pneumoniae, Lactobacillus casei, MRSA, Neisseria gonorrhoeae, Neisseria subflava, Porphyromonas ginigvalis, Prevotella nigrescens, Propionibacterium acnes, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus (MRSA and MSSA), Staphylococcus epidermidis, Staphylococcus haemolyticus, Stenotrophomonas maltophilia, Streptococcus mutans, Streptococcus oralis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus zooepidemicus, Tannerella forsythia, Trichophyton rubrum, Veillonella dispar. WHO Priority organisms. Other- please ask.