Oral Care and Waterline Systems

Perfectus Biomed have experience in oral biofilm assays, antimicrobial polymers and bacteriostatic and bactericidal differentiation.

The concept of chronic microbial infections, specifically the term biofilm, entered the scientific and microbiological world in relation to dental biofilms, an example of which is plaque. 

Biofilms are problematic for the dental industry as dental unit waterlines can become contaminated with environmental microorganisms, forming biofilms that provide nutrients and protection to the resident microorganisms. Due to the force of liquid through the waterlines, bacteria may detach from the walls and contaminate the water stream, putting patients and healthcare professionals at risk of infection. 

Biofilm removal techniques and products have also undergone a large amount of research within the dental world. Removal techniques include teeth brushing and flossing, as well as biofilm disruption agents found in toothpastes and mouthwashes. Despite the high body of knowledge in the dental industry, there is still a lot of research and development required. This includes the development of products that disrupt and remove oral biofilms as well as anti-microbial products to prevent and remove biofilm material. Anti-microbial products can also prevent chronic microbial build-up in dental unit waterlines and on dental surfaces equipment. Perfectus Biomed assess the biofilm removal and prevention properties of  products to BS EN ISO 16954 methodology.

Perfectus Biomed offer a range of dental testing methods including oral biofilm assays and ISO 16954: Dental Unit Waterlines Testing. GLP compliant testing is also available. 

Please contact us if you would like to discuss test methods that suit your requirements.

test methodologies that we offer:

  • ISO 16954: Test methods for dental unit waterline biofilm removal and prevention
  • Single species oral biofilm assays
  • Multi-species oral biofilm assays
  • Assessment of odour reduction properties of products

experience working with:

  • Toothpastes
  • Mouth washes
  • Dental unit water lines
  • Cleansers
  • Antimicrobial peptides

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans

Candida auris
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

Medical Devices

We can use the PMM to test for bacterial adhesion to and host cell toxicity of experimental devices.

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Urogenital Health

Since much of the human genital tract is composed of mucosal tissue, the PMM can be used for predictive preclinical testing of compounds or products related to urogenital health.

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Inflammation and Biocompatibility

The PMM offers a unique way to investigate inflammatory cytokine concentrations in a cost-effective manner. We can perform ELISAs and multiplex assays to determine whether experimental compounds are pro- or anti-inflammatory.

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Infectious Diseases

Mucosal tissue is an ideal environment for microbial growth, making the PMM an excellent application for testing the efficacy of products to treat infectious disease pathogens. We can test for efficacy against planktonic and biofilm infections with this model.

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Get in Touch

We are happy to help with your microbiological requirements and with any other queries you may have.

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Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

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