CELL LINE IDENTIFICATION:

Authentication of cell lines and other key biological materials enhances reproducibility of research findings through increased scientific rigor and transparency. Authentication plans are therefore required for many grant applications to ensure authenticity of the cell line throughout multiple timepoints during continuous use in projects.

Best practice guidelines recommend cell line testing:

• Prior to submitting a paper for review or beginning a new grant
• To prevent cross-contamination
• If cells give erratic and unreproducible results
• If a change in morphology or growth characteristics are observed
• When creating a new cell line
• When receiving cells from a non-accredited laboratory/provider
• When creating cell stocks for storage

What we offer:

Perfectus Biomed work to the ATCC ASN-0002-2011 standard, with the ability to test at 24 loci using Promega’s Geneprint 24 system to prevent misidentification of closely related cell lines. The 24 loci that will be tested include: TH01, TPOX, vWA, Amelogenin (for gender identification), CSF1PO, D16S539, D7S820, D13S317 and D5S818. These are the nine markers as outlined within ASN-0002-2011. We provide clients with a signed report that covers the basic requirement for grant/journal submissions. Reports will include the electropherogram analysis and interpretation of the Short Tandem Repeat (STR) profile compared to the published standard profile, where required. 

 PATHOGEN DETECTION:

Mycoplasma can attain high densities within cell cultures with an absence of visible morphological changes. Undetected contamination by mycoplasma species or other pathogens invalidate work, and so regular sterility testing is critical for continuous cell lines.

Pathogen detection testing is recommended:

• When creating a master cell bank or working cell bank
• Before beginning a new series of experiments
• When the cell line behaves abnormally
• Every two months that the cell culture is actively growing
• Prior to publication of experimental data

SAMPLE REQUIREMENTS:

For pathogen detection, cell line authentication and STR analysis we accept the following formats of cell lines:

Fresh or frozen cell pellets

• Please provide a minimum cell number of 1×10⁶ cells/mL.
• Fresh cells must be washed in appropriate buffer to remove media/trypsin traces. Please specific storage media used when filling out our Submission Form.
• When overnight shipping samples please ship with icepacks or dry ice for longer shipping periods.

Dried cell pellet

• Please provide a minimum cell number of 1×10⁶ cells/mL.
• Fresh cells must be washed in appropriate buffer to remove media/trypsin traces. Please specific storage media used when filling out our Submission Form.
• Please ensure the tube has no residual liquid using Vacuum pumps.
• Dry samples may be shipped at ambient temperatures.

Genomic DNA

• Extracted DNA is also accepted at a reduced cost to cell pellets. Please note the DNA concentration of the sample on the Submission Form.
• Please provide DNA at a minimum concentration >10ng/µl with a total volume of 20µl.
• If you are not able to meet the requirements stated please contact to see if we can accommodate your specific sample size.
• Extracted DNA samples may be shipped at ambient temperatures.

Viral primers include:

Xenotropic Virus Testing
XMRV

Pre-XMRV 1

Pre-XMRV2

XMLV

Bxv-1

MLV N417

Biofilm gene expression for:

S. aureus NCTC 8325

C. albicans ATCC MYA 2867

P. aeruginosa NCIMB 10421 (PA14)