Why consider cell banking services?
A proficient researcher understands the problems associated with maintaining a cell line in continuous or extended cell culture. We understand our clients need to ensure cell and data integrity is maintained throughout a controlled sample management lifecycle. Taking care of cell banks internally means your team’s time and resources are taken away from research and maintaining strict cell line quality control can be challenging when working to tight project timelines. Maintaining cell lines in continuous culture comes with significant staff, equipment, and consumables costs. Our complete cell banking service, in collaboration with Cryoniss, offers an agile end-to end solution that includes access to your cells when you need them, long term cryogenic storage and worldwide transportation.
What are the risks of cell line misidentification?
Cell line quality control in line with the ATCC-ASN-0002-2021 standard is paramount when handling cell cultures. The potential risks factors of maintaining cell lines in continuous or extended culture include cross contamination, cell line misidentification and genotypic instability. “Cross-contamination was first recognised in the 1950s but, disturbingly, remains a serious issue today.1” (Capes-Davis, et al, 2010) It was during some of the first studies carried out with HeLa cells that led to the discovery that vigorous lines could contaminate and overgrow slower growing cultures. Mycoplasma are a common contaminate of cell lines, they have an ability to attain high densities within cell cultures with an absence of visible morphological changes. “Although no exact numbers are known, the extent of cell line misidentification is estimated between one fifth and one third of all cell lines.”2 (Horbach & Halffman, 2017)
What is the process of cell banking?
The implementation of an outsourced cell bank not only ensures peace of mind due to minimised potential risk factors, but also allows you to access your cell bank when you need them. On initial arrival into the laboratory a new cell culture will be regarded as a potential source of contamination, and we will conduct cell line quality control testing to ensure cell bank starting material is correctly identified. Once checked, cell banks can be built into master and working cell banks. Then before any cell line leaves the lab, this maybe to be stored in cryogenic storage or transported globally, cell line QC testing will be completed once more. This end-to-end service offering is customisable to suit your needs.
This new service offering launches a collaboration with Cryoniss, that aligns a unique blend of scientific expertise, a mutual focus on quality and shared values and ethics. Sonia Houghton, co-founder, and CEO of Cryoniss said “This latest collaboration gives us the ability, to support our customers with the generation of high quality cell banks, providing them assurance that their cells are free from contaminants and allowing them to focus on the value-add science – allowing us to respond to our customers’ needs in an agile and responsive way and giving them added confidence that the samples they’re working with will produce excellent, reliable data.”
To find out more about how our cell banking services can benefit your business, please get in touch by emailing info@perfectusbiomed.com or solutions@cryoniss.com.
References
- https://onlinelibrary.wiley.com/doi/10.1002/ijc.25242
- https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186281#:~:text=Although%20no%20exact%20numbers%20are,used%20infrequently%20%5B15%5D