Microbiology Research

Perfectus Biomed offer standard and customised microbiology.

We combine an academically-based approach with the professionalism, speed and accreditation of industry. We are ISO 9001 certified, offer UKAS accredited ISO 17025 biofilm test methods and are GLP compliant.

Perfectus Biomed work closely with our clients to understand their requirements, offering a tailored method design to suit your testing needs. We recognise the difference between generating data for regulatory, marketing and internal purposes.

Perfectus Biomed support clients with their data generation portfolio in order to support regulatory processes such as 510(k) submissions and Pre-IND applications.

Customised Microbiology

Perfectus Biomed can provide method adaptations to suit your requirements. Customised test methods are required when standard methods are not appropriate for the novel product, test methods are not available and also when internal test methods require independent validation.

Examples:

  • A novel test product may be in an inappropriate state for the test method e.g. a solid state antimicrobial could not be tested as part of an assay designed for testing liquid antimicrobials.
  • An assay designed for testing wound dressings may not account for the variation in dressing layers/structures and absorbencies.
  • The unique selling feature of a product may not be demonstrated using standard test methodologies.

How Perfectus Biomed can help:

  • Our services include extensive consultation with clients in order to ensure that we understand the microbiological question you are seeking to answer as well as the intended data use.
  • We tailor our method design so that it is appropriate for the testing of your product.
  • All assays include appropriate positive and negative controls and suitable repeats in order to ensure validity of the data.
  • Our PhD microbiologists run your projects to ensure a detailed understanding of your research.

Microscopy Techniques

Scanning Electron Microscopy (SEM):

An electron microscope is used to investigate the structural characteristics of a range of biological and inorganic specimens, from microorganisms and cells to metals and crystals. Within microbiology, electron microscopy assesses the structure of unicellular organisms within microbial biofilms, using electron beams as their illumination source to provide high resolution images.

Scanning electron microscopy images provide visualisation of material topography of a sample. They use a focused electron beam to scan a rectangular area of the specimen. Perfectus Biomed use SEMs within our biofilm testing methods, such as ISO 16954: test method for dental unit waterline biofilm treatment, to provide a visual follow-up to treatments. SEMs can be used to qualitatively assess microorganism and biofilm coverage on the surface of dental unit waterline tubing.

LIVE/DEAD staining:

This technique determines cell viability and cell vitality, by staining cells with a fluorescent dye. In live cells, the dye cannot penetrate cell membranes and so it reacts with cellular proteins on the cell surface. This results in dim staining of the live cell. In dead cells, the reactive dye permeates the damaged cell membranes, staining both the interior and exterior proteins, which results in a more intense staining. This technique is used at Perfectus Biomed to determine cell viability following treatment.

Confocal Laser Scanning Microscopy (CLSM):

Confocal microscopes use a pin-hole focused laser to capture three dimensional images of a sample at different depths. This technique is used to obtain serial optical sections (z-sections) of a sample, which can be stacked on top of each other (z-stacking) to create a 2D image or reconstruct a 3D image of the sample. We use this technique at Perfectus Biomed with the SYTO 64 stain, which exhibits a bright red fluorescence upon binding to nucleic acids. Z-sections of samples are imaged to measure biofilm coverage of the sample.

Phase Contrast Microscopy:

Phase contrast microscopy is used in cell culture and live cell imaging to observe cells in their natural state without fixation or labelling. This technique transforms phase differences of light into differences in amplitude of light, i.e. creating light and dark areas.

Gray Metal Microscope

 

Please contact us if you would like to discuss our services.

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

Get in Touch

We are happy to help with your microbiological requirements and with any other queries you may have.

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Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

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