Cell Culture and Viral Testing

Perfectus Biomed specialise in cell culture and viral testing. This includes biocompatibility testing, virucidal efficacy testing and viral barrier testing, to name a few.

Biocompatibility testing is essential to support the biological evaluation of medical devices. Perfectus Biomed use in vitro wound models and also cell models to aid biocompatibility assessments using ISO 10993 guidance. We measure parameters such as cytotoxicity, hemocompatibility, irritation, corrosion and proliferation viability.

Virucidal efficacy testing is important for assessing the activity of a variety of products against viruses. These products typically include both household and industrial disinfectants. Virucidal efficacy testing can be carried out by using either suspension tests or hard surface carrier tests. Perfectus Biomed also offer viral barrier testing for wound dressings and many other materials.

We will work with you to help you understand the benefits of the different cell and viral models. We will therefore help you to choose a test method that is most suitable for both you and your product.

 

Cell Culture and Viral Testing:

Please see the below list of cell culture assays and viral assays that Perfectus Biomed can offer. Please contact us if you would like to discuss a test method with us in more detail.

Cell culture assays include:

  • Biocompatibility – ISO 10993-3 Biological evaluation of medical devices – Part 3: Tests for genotoxicity
  • Biocompatibility – ISO 10993-4 Biological evaluation of medical devices – Part 4: Selection of tests for interaction with blood
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Direct contact
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Indirect contact
  • USP 87 – Cytotoxicity
  • In vitro scratch test
  • Gel contraction assays
  • EpiSkin Reconstructed Human Epidermis – Skin Irritation Assay
  • EpiSkin Reconstructed Human Epidermis – Skin Corrosion Assay

 

Viral assays include:

  • AATCC 100 – Assessment of Antibacterial Finishes on Textile Materials, Modified for Viruses
  • ASTM E1052 – Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension
  • ASTM E1053 – Standard Test Method to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate, Nonporous Environmental Surfaces
  • EN 14476 – Chemical Disinfectants and Antiseptics – Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area
  • Pre-Saturated Towelettes Virucidal Effectiveness Test
  • Viral barrier testing

 

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae
Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae
Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar
WHO Priority organisms.

Other- please ask.