Cell Culture and Viral Testing

Perfectus Biomed specialise in cell culture and viral assays.

Biocompatibility testing is essential to support the biological evaluation of medical devices. Perfectus Biomed use in vitro wound models and cell models to aid biocompatibility assessments, measuring parameters such as cytotoxicity, hemocompatibility, irritation, corrosion, proliferation  viability.

Virucidal efficacy testing can be carried out using suspension tests or hard surface carrier tests. These methods can be used to assess a range of products for example household and industrial disinfectants. Perfectus Biomed also offer viral barrier testing for wound dressings and other materials.

We will work with you to help you understand the benefits of the different cell and viral models and to choose a test method that is most suitable for your product.

 

Cell culture assays include:

  • Biocompatibility – ISO 10993-3 Biological evaluation of medical devices – Part 3: Tests for genotoxicity
  • Biocompatibility – ISO 10993-4 Biological evaluation of medical devices – Part 4: Selection of tests for interaction with blood
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Direct contact
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Indirect contact
  • USP 87 – Cytotoxicity
  • In vitro scratch test
  • Gel contraction assays
  • EpiSkin Reconstructed Human Epidermis – Skin Irritation Assay
  • EpiSkin Reconstructed Human Epidermis – Skin Corrosion Assay

 

Viral assays include:

  • AATCC 100 – Assessment of Antibacterial Finishes on Textile Materials, Modified for Viruses
  • ASTM E1052 – Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension
  • ASTM E1053 – Standard Test Method to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate, Nonporous Environmental Surfaces
  • EN 14476 – Chemical Disinfectants and Antiseptics – Quantitative Suspension Test for the Evaluation of Virucidal Activity in the Medical Area
  • Pre-Saturated Towelettes Virucidal Effectiveness Test
  • Viral barrier testing

 

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii, Actinomyces naeslundii, Adenovirus (human), Aspergillus brasiliensis, Aspergillums niger, Aureobasidium pullulans, Bacillus cereus, Bacillus subtilis, Camplylobacter jejuni, Candida albicans, Clostridium difficile, Corynebacterium minutissimum, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium (VRE), Enterococcus hirae, Escherichia coli, Fusobacterium nucleatum, Haemophilus influenzae, Heliocbacter pylori, Halomonas magadiensis, Klebsiella pneumoniae, Lactobacillus casei, MRSA, Neisseria gonorrhoeae, Neisseria subflava, Porphyromonas ginigvalis, Prevotella nigrescens, Propionibacterium acnes, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus (MRSA and MSSA), Staphylococcus epidermidis, Staphylococcus haemolyticus, Stenotrophomonas maltophilia, Streptococcus mutans, Streptococcus oralis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus zooepidemicus, Tannerella forsythia, Trichophyton rubrum, Veillonella dispar. WHO Priority organisms. Other- please ask.