Cell Culture and Testing

Perfectus Biomed specialise in cell culture to support the biological evaluation of your product.

We use in vitro wound models and cell models to aid biocompatibility assessments using ISO 10993 guidance. We measure parameters such as cytotoxicity (USP 87), hemocompatibility, irritation, corrosion and proliferation viability. Testing to GLP is available.

Perfectus Biomed work closely with our clients, from study design to production of the scientific report.

We typically offer cell services to support our clients for one of three reasons:

1. Research and Development: product development, screening and pre-clinical data.

2. Biocompatibility and Sfety/regulatory Requirements: legal requirements and label claims.

3. Marketing: evidence to support the product on the market by highlighting competitive advantages, differentiations and USPs.

Biocompatibility and Safety Testing

  • In vitro cytotoxicity: BS EN ISO 10993-5
  • In vitro skin irritation using 3D reconstructed human epidermis: OECD 439
  • In vitro skin corrosion using 3D reconstructed human epidermis: OECD TG431

Regulatory and Label Claims

  • In vitro virucidal activity: BS EN 14476
  • Macrophage activation assay (assessment for inhibition)
  • Colonised skin model: in-house method

Marketing and Research & Development

  • Scratch wound model: in-house method
  • Conference abstracts and presentations

We help companies to accelerate their pipeline, expand product claims and highlight competitive advantages.

Cell Culture Testing:

Please see the below list of cell culture assays that Perfectus Biomed can offer. Please contact us if you would like to discuss a test method with us in more detail.

  • Biocompatibility – ISO 10993-3: Tests for genotoxicity
  • Biocompatibility – ISO 10993-4: Interaction with blood
  • Biocompatibility – ISO 10993-5:  in vitro cytotoxicity – Direct contact
  • Biocompatibility – ISO 10993-5:  in vitro cytotoxicity – Indirect contact
  • USP 87 – Cytotoxicity
  • In vitro scratch wound closure
  • Gel contraction assays
  • EpiSkin Reconstructed Human Epidermis – Skin Irritation Assay
  • EpiSkin Reconstructed Human Epidermis – Skin Corrosion Assay
  • Macrophage activation
  • Co-culture with monolayer
  • MMP concentration assessments
  • Acne models
  • Phenotypic screening of drugs
  • Basic gut epithelial models
  • Cancer invasion assays
  • Molecular analysis – pathway targeting
  • Genotoxicity
  • Cytokine measurement
  • Growth factor measurement
  • Measurements of extracellular matrix components
  • Protease measurement
  • Colonised skin models
  • Standard anti-viral testing
  • Cell proliferation/viability
  • Collagen production
  • Wound closure in 3D skin
  • Biofilm-wound models
  • Antiviral fogging
  • Corneal model (bacterial adhesion, eye irritation)
  • Oral models (oral care, bacterial adhesion)
  • Vaginal models (feminine hygiene care/bacterial adhesion)
  • Diabetic models
  • Gut microbiome models
  • Biocidal activity against parasites

Human Cell Types:                                                            

Human dermal fibroblasts (normal, adult)

Human epidermal keratinocytes (normal, neonatal)

HeLa (epithelial, adenocarcinoma)

H1-HeLa (epithelial, adenocarcinoma, viral susceptibility)

HEK-293 (epithelial, contain adenovirus DNA)

Animal Cell Types:

Vero (epithelial, normal)

L-929 (fibroblast, normal)

RAW 264.7 (macrophage, Abelson murine leukemia virus-induced tumor)

MDCK (epithelial, norma

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

Get in Touch

We are happy to help with your microbiological requirements and with any other queries you may have.

<img src="http://perfectusbiomed.com/wp-content/uploads/2018/08/logonew.png"><br />Contact Us

Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

Get in Touch