Cell Culture and Testing

Perfectus Biomed specialise in cell culture to support the biological evaluation of your product.

We use in vitro wound models and cell models to aid biocompatibility assessments using ISO 10993 guidance. We measure parameters such as cytotoxicity (USP 87), hemocompatibility, irritation, corrosion and proliferation viability. GLP compliant testing is also available.

Perfectus Biomed work closely with our clients, from study design to production of the scientific report.

Human Cell Types:                                                            

Human dermal fibroblasts (normal, adult)

Human epidermal keratinocytes (normal, neonatal)

HeLa (epithelial, adenocarcinoma)

H1-HeLa (epithelial, adenocarcinoma, viral susceptibility)

HEK-293 (epithelial, contain adenovirus DNA)

Animal Cell Types:

Vero (epithelial, normal)

L-929 (fibroblast, normal)

RAW 264.7 (macrophage, Abelson murine leukemia virus-induced tumor)

MDCK (epithelial, normal)


We help companies to accelerate their pipeline, expand product claims and highlight competitive advantages.

Cell Culture Testing:

Please see the below list of cell culture assays that Perfectus Biomed can offer. Please contact us if you would like to discuss a test method with us in more detail.

Cell culture assays include:

  • Biocompatibility – ISO 10993-3 Biological evaluation of medical devices – Part 3: Tests for genotoxicity
  • Biocompatibility – ISO 10993-4 Biological evaluation of medical devices – Part 4: Selection of tests for interaction with blood
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Direct contact
  • Biocompatibility – ISO 10993-5 Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity – Indirect contact
  • USP 87 – Cytotoxicity
  • In vitro scratch test
  • Gel contraction assays
  • EpiSkin Reconstructed Human Epidermis – Skin Irritation Assay
  • EpiSkin Reconstructed Human Epidermis – Skin Corrosion Assay
  • Macrophage activation
  • Cytokine measurement
  • Growth factor measurement
  • Measurements of extracellular matrix components
  • Protease measurement
  • Colonised skin models

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

Get in Touch

We are happy to help with your microbiological requirements and with any other queries you may have.

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Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

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