Biofilm Testing

Perfectus Biomed are specialists in designing, running and interpreting biofilm assays.

Understanding the problem of biofilms

Microorganisms such as bacteria, fungi and yeast can exist as free floating planktonic entities or they can attach to a surface or one another and form a microbial biofilm.

When microorganisms live beneath a biofilm they are significantly less susceptible to antimicrobial agents (and the host’s immune system).

In nature 80% of bacteria exist within a biofilm.

Standard testing focuses almost exclusively on planktonic bacteria, as such current standard testing may underestimate the quantity of active agent required to effectively remove a microbial biofilm.

What do we offer?

We will work with you to help you understand the benefits of the different models and to choose a test method that is most suitable for your product.

We will test your product using either commercially available biofilm models or adapt/design a biofilm model that suites your requirement.

Examples of available test models (please contact us if you do not see the model you are interested in on the list below).

  • Microtitre plate assay
  • Calgary biofilm device: Minimum Biofilm Eradication Concentration (MBEC) and Minimum Biofilm Inhibition Concentration (MBIC)
  • CDC reactor model (ASTM E2871 and ASTM E2562)
  • Drip flow reactor model (ASTM E2647)
  • Colony drip flow reactor model
  • Porcine skin explant drip flow model
  • Flow cell reactor model
  • Models are available using static or continuous flow systems

List of UKAS ISO 17025:2005 accredited biofilm test methodologies:


SOP 537: CDC reactor model

SOP 538: Drip flow reactor model

Find our schedule of accreditation here.


Common variations on these assays include

  • Multiple range of proven biofilm forming pathogens are available for testing
  • Single and multi-species assays available as requested
  • Biofilm testing using anaerobic bacteria
  • Biofilm testing using yeast and fungi

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii, Actinomyces naeslundii, Adenovirus (human), Aspergillus brasiliensis, Aspergillums niger, Aureobasidium pullulans, Bacillus cereus, Bacillus subtilis, Camplylobacter jejuni, Candida albicans, Clostridium difficile, Corynebacterium minutissimum, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium (VRE), Enterococcus hirae, Escherichia coli, Fusobacterium nucleatum, Haemophilus influenzae, Heliocbacter pylori, Halomonas magadiensis, Klebsiella pneumoniae, Lactobacillus casei, MRSA, Neisseria gonorrhoeae, Neisseria subflava, Porphyromonas ginigvalis, Prevotella nigrescens, Propionibacterium acnes, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella dysenteriae, Staphylococcus aureus (MRSA and MSSA), Staphylococcus epidermidis, Staphylococcus haemolyticus, Stenotrophomonas maltophilia, Streptococcus mutans, Streptococcus oralis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus zooepidemicus, Tannerella forsythia, Trichophyton rubrum, Veillonella dispar. WHO Priority organisms. Other- please ask.