Perfectus Biomed are leading the market with accredited biofilm methods.

Microorganisms such as bacteria, fungi and yeast can exist as free floating planktonic entities or they can attach to a surface (or one another) to form a microbial biofilm. In nature, 80% of bacteria exist within a biofilm. When microorganisms live beneath a biofilm, they are significantly less susceptible to antimicrobial agents (and the host’s immune system).

Standard microbiological testing focuses almost exclusively on planktonic bacteria. As such, current standard testing may underestimate the quantity of active agent required to effectively remove a microbial biofilm.

The type of biofilm testing that we offer?

Perfectus Biomed will work with you to help you understand the benefits of different biofilm testing models. We will then choose a test method that is most suitable for your product, using either commercially available biofilm models or adapting/designing a biofilm model that suites your requirement.

  • SOP 536: Standard test method for testing disinfectant efficacy against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans biofilms using the Minimum Biofilm Eradication Concentration (MBEC) assay.
  • SOP 537: Standard test method for the quantification of biofilm growth with low shear using the CDC biofilm reactor.
  • SOP 538: Standard test method for the quantification of biofilm grown using the Drip Flow biofilm reactor with low shear and continuous flow.
  • SOP 555: Quantification of Biofilm Grown with Low Shear Using the CDC Biofilm Reactor in a Batch System C.Albicans

GLP compliant testing is also available. 

Please contact us if you do not see the model you are interested in on the list below.

Examples of ISO 9001 METHODS:

  • Colony Drip Flow reactor model
  • Porcine skin explant drip flow model
  • Single and multi-species biofilm assays
  • Bacterial and fungal biofilm assays
  • Microtitre plate assay
  • Flow cell reactor model

Models are available using static or continuous flow systems.

examples of ISO 17025 accredited methods:

  • SOP 536: Minimum Biofilm Eradication Concentration (MBEC) assay
  • SOP 537: CDC biofilm reactor assay with low shear using Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans
  • SOP 538: Drip flow biofilm reactor assay with low shear and continuous flow

Find our schedule of accreditation here.

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

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We are happy to help with your microbiological requirements and with any other queries you may have.

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Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

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