Biofilm Testing

Perfectus Biomed are specialists in designing, running and interpreting biofilm assays. We offer both standard and customised biofilm testing models.

Understanding the problem of biofilms

Microorganisms such as bacteria, fungi and yeast can either exist as free floating planktonic entities or they can attach to a surface (or one another) to form a microbial biofilm. In nature, 80% of bacteria exist within a biofilm.

When microorganisms live beneath a biofilm, they are significantly less susceptible to antimicrobial agents (and the host’s immune system).

Standard biofilm testing focuses almost exclusively on planktonic bacteria, As such, current standard testing may underestimate the quantity of active agent required to effectively remove a microbial biofilm.

The type of biofilm testing that we offer?

Perfectus Biomed will work with you to help you understand the benefits of the different biofilm testing models. We will then choose a test method that is most suitable for your product.

We will test your product using either commercially available biofilm models or adapt/design a biofilm model that suites your requirement.

Please contact us if you do not see the model you are interested in on the list below.

Examples of available test models:

  • Microtitre plate assay
  • Calgary biofilm device: Minimum Biofilm Eradication Concentration (MBEC) and Minimum Biofilm Inhibition Concentration (MBIC)
  • CDC reactor model (ASTM E2871 and ASTM E2562)
  • Drip flow reactor model (ASTM E2647)
  • Colony drip flow reactor model
  • Porcine skin explant drip flow model
  • Flow cell reactor model
  • Models are available using static or continuous flow systems

List of UKAS ISO 17025:2005 accredited biofilm test methodologies:


SOP 537: CDC reactor model

SOP 538: Drip flow reactor model

Find our schedule of accreditation here.


Common variations on these assays include

  • Multiple range of proven biofilm forming pathogens are available for testing
  • Single and multi-species assays available as requested
  • Biofilm testing using anaerobic bacteria
  • Biofilm testing using yeast and fungi

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae
Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae
Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar
WHO Priority organisms.

Other- please ask.