Perfectus Biomed are leading the market with accredited biofilm methods.

Microorganisms such as bacteria, fungi and yeast can exist as free floating planktonic entities or they can attach to a surface (or one another) to form a microbial biofilm. In nature, 80% of bacteria exist within a biofilm. When microorganisms live beneath a biofilm, they are significantly less susceptible to antimicrobial agents (and the host’s immune system). We offer a variety of biofilm testing methods, such as the Single Tube Method, the Drip Flow Biofilm Reactor, the CDC Biofilm Reactor and the Minimum Biofilm Eradication Concentration (MBEC). 

Standard microbiological testing, however, focuses almost exclusively on planktonic bacteria. As such, current standard testing may underestimate the quantity of active agent required to effectively remove a microbial biofilm.

The type of biofilm testing that we offer?

Perfectus Biomed will work with you to help you understand the benefits of different biofilm testing models. We will then choose a test method that is most suitable for your product, using either ISO 17025 accredited biofilm models or adapting/designing a biofilm model that suits your requirement.

GLP compliant testing is also available. 

Please contact us if you do not see the model you are interested in on the list below.

examples of ISO 17025 accredited methods:

Minimum Biofilm Eradication Concentration (MBEC)

The Minimum Biofilm Eradication Concentration (MBEC) assay is a high throughput screening model. The MBEC assay evaluates biofilm grown under batch conditions (no flow of nutrients into or out of an individual well). The model can be used to determine the efficacy of multiple antimicrobial products simultaneously at multiple concentrations against pre-formed biofilms.

SOP 536: Standard test method for testing disinfectant efficacy against Pseudomonas aeruginosa and Staphylococcus aureus biofilms using the Minimum Biofilm Eradication Concentration (MBEC) assay modified from ASTM E2799 standard method.

CDC Biofilm Reactor

The CDC Biofilm Reactor model develops reproducible biofilms on 24 individual coupon surfaces under flow. The coupon materials used in the model are interchangeable and can be chosen to help simulate the use of a product. Examples include polycarbonate, copper, ceramic, stainless steel and glass. The reactor can be tested in a batch system or can include addition of fresh nutrients during incubation to simulate real-world environments in which biofilms develop.

SOP 537/555: Quantification of biofilm growth with low shear using the CDC biofilm reactor (Pseudomonas aeruginosa and Staphylococcus aureus and Candida albicans) modified from ASTM E2562 standard method.

Drip Flow Biofilm Reactor

The Drip Flow Biofilm Reactor is used to reproduce biofilms on glass coupons under defined conditions suitable for efficacy testing. The biofilms represent generalised situations where biofilm exists at the air/liquid interface with a continuous flow of nutrients under low fluid shear. The Drip Flow Reactor can be used for growing and characterising multi-species biofilms. Based on the growth requirements of the organism, the method can be optimised by changing the operational parameters.

SOP 538: Standard test method for the quantification of biofilm grown using the Drip Flow biofilm reactor with low shear and continuous flow modified from ASTM E2647 standard method.

Single Tube Method

The Single Tube Method is used to determined the efficacy of biocides against pre-formed biofilms, testing test water soluble powders or liquid formations on hard or non-porous surfaces in which biofilm can grow. The Single Tube Method is used to obtain biofilm removal claims, which demonstrate a products’ ability to remove biofilms. In order to register an antimicrobial product for a public health biofilm removal claim, the Environmental Protection Agency (EPA) require efficacy data to be submitted under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA).

SOP 575: Test method for testing disinfectant efficacy against bacterial biofilm grown for 48 hours following the principles of ASTM E2871-19 in the CDC biofilm reactor using the single tube method.

Examples of ISO 9001 METHODS:

  • Colony Drip Flow reactor model
  • Porcine skin explant drip flow model
  • Porcine lung model for cystic fibrosis treatment
  • Single and multi-species biofilm assays
  • Bacterial and fungal biofilm assays
  • Microtitre plate assay
  • Flow cell reactor model

Models are available using static or continuous flow systems.

Our library of test organisms includes: 

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans

Candida auris
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae

Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae

Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar

WHO Priority organisms.
ESKAPE pathogens.

Other- please ask.

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We are happy to help with your microbiological requirements and with any other queries you may have.

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Perfectus Biomed

Perfectus Biomed, a UKAS accredited microbiological contract testing company, represents the new wave of scientific businesses that are proud to call Daresbury home.

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