Dental Biofilm Test Methods

The concept of chronic microbial infections, specifically the term biofilm, entered the scientific and microbiological world in relation to dental biofilms. An example of a dental biofilm is plaque. Plaque is the most well-known and researched area of dental biofilm research with well understood causes and consequences.

Biofilm removal techniques and products have also undergone a large amount of research within the dental world. Removal techniques include teeth brushing and flossing, as well as biofilm disruption agents found in toothpastes and mouthwashes.

Despite the high body of knowledge in the dental industry, there is still a lot of research and development required. This includes the development of products that disrupt and remove oral biofilms as well as anti-microbial products. Anti-microbial products can prevent and remove biofilm material. They can also prevent chronic microbial buildup in dental unit waterlines and on dental surfaces equipment.

Perfectus Biomed offer a range of dental testing methods including oral biofilm assays and ISO 16954: Dental Unit Waterlines Testing.

Please contact us if you would like to discuss test methods that suit your requirements.


Perfectus Biomed’s Experience:

We offer a range of test methodologies which include:

  • Single species oral biofilm assays
  • Multi-species oral biofilm assays
  • ISO 16954: Test methods for dental unit waterline biofilm treatment


Our testing experience includes working with:

  • Toothpastes
  • Mouth washes
  • Dental unit water lines
  • Cleansers
  • Antimicrobial peptides

Our library of test organism includes…

Choose from our library of test organism including standard reference strains, clinical isolates and environmental isolates.

Single or mixed species assays containing:

Acinetobacter baumannii
Actinomyces naeslundii
Adenovirus (human)
Aspergillus brasiliensis
Aspergillums niger
Aureobasidium pullulans
Bacillus cereus
Bacillus subtilis
Camplylobacter jejuni
Candida albicans
Clostridium difficile
Corynebacterium minutissimum
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium (VRE)
Enterococcus hirae
Escherichia coli
Fusobacterium nucleatum
Haemophilus influenzae
Heliocbacter pylori
Halomonas magadiensis
Klebsiella pneumoniae
Lactobacillus casei
Neisseria gonorrhoeae
Neisseria subflava
Porphyromonas ginigvalis
Prevotella nigrescens
Propionibacterium acnes
Proteus mirabilis
Pseudomonas aeruginosa
Salmonella enteritidis
Shigella dysenteriae
Staphylococcus aureus (MRSA and MSSA)
Staphylococcus epidermidis
Staphylococcus haemolyticus
Stenotrophomonas maltophilia
Streptococcus mutans
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus zooepidemicus
Tannerella forsythia
Trichophyton rubrum
Veillonella dispar
WHO Priority organisms.

Other- please ask.